Increasing the intensity of illumination laser risks excessive bleaching or other damage to the specimen of interest, especially for experiments in which comparison of fluorescence brightness is required.
2.
These methods are all, to some extent, functional at reducing the unevenness of illumination however they all reduce intensity of illumination and alter the range of wavelengths of light which reach the sample.
3.
Once exposure time has exceeded about 1 second for film or 30 seconds for paper, one must compensate for the breakdown in linear response of the film / paper to intensity of illumination by using longer exposures.
4.
One can think of luminous flux ( measured in lumens ) as a measure of the total " amount " of visible light present, and the illuminance as a measure of the intensity of illumination on a surface.
5.
He gave a proof of the mean speed theorem stating that " the latitude of a uniformly difform movement corresponds to the degree of the midpoint " and used this method to study the quantitative decrease in intensity of illumination, stating that it was not linearly proportional to the distance, but was unable to expose the Inverse-square law, which was postponed nearly 250 years until its discovery by Johannes Kepler in 1604.
6.
He gave a proof of the mean speed theorem stating that " the latitude of a uniformly difform movement corresponds to the degree of the midpoint " and used this method to study the quantitative decrease in intensity of illumination in his " Summa logic?et philosophi?naturalis " ( ca . 1349 ), stating that it was not linearly proportional to the distance, but was unable to expose the Inverse-square law.
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